Respiration and Active Sodium Transport of Isolated Toad Bladder

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Respiration and active sodium transport of isolated toad bladder.

Preparation of Tissue-Studies were performed on the isolated urinary bladder of the toad, Bujo marinus. Animals were pithed and the two halves of the bladder rapidly removed through a ventral incision. The tissue was rinsed in Ringer’s solution before use. For the study of the oxygen consumption of various toad tissues, small intestine, stomach, heart, ventral abdominal skin, and skeletal muscl...

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Active Sodium Transport by the Isolated Toad Bladder

Studies were made of the active ion transport by the isolated urinary bladder of the European toad, Bufo bufo, and the large American toad, Bufo marinus. The urinary bladder of the toad is a thin membrane consisting of a single layer of mucosal cells supported on a small amount of connective tissue. The bladder exhibits a characteristic transmembrane potential with the serosal surface electrica...

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Effect of purified staphylococal alpha toxin on active sodium transport and aerobic respiration in the isolated toad bladder.

Purified staphylococcal alpha toxin was found to inhibit the active transport of sodium across the isolated toad bladder when applied to the serosal but not to mucosal surface. Heating or the addition of specific antitoxin abolished this effect. Low temperatures reduced this activity significantly. Application of vasopressin to the bladder serosa shortly after toxin resulted in only weak and tr...

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Stimulation of Active Sodium Transport by the Isolated Toad Bladder with Aldosterone

The tubular reabsorption of sodium in the mammalian kidney is stimulated after administration of aldosterone (1-3). The transport of sodium from the tubular lumen to the interstitial compartments, across the cells lining the renal tubule, represents an active process (4, 5), and one is therefore led to conclude that this process is influenced by aldosterone. However, the failure to observe a so...

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Stimulation of active sodium transport by the isolated toad bladder with aldosterone in vitro.

The tubular reabsorption of sodium in the mammalian kidney is stimulated after administration of aldosterone (1-3). The transport of sodium from the tubular lumen to the interstitial compartments, across the cells lining the renal tubule, represents an active process (4, 5), and one is therefore led to conclude that this process is influenced by aldosterone. However, the failure to observe a so...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1959

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)70061-6